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KMID : 0880220140520050435
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Journal of Microbiology
2014 Volume.52 No. 5 p.435 ~ p.440
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Comparison of JEV Neutralization Assay Using Pseudotyped JEV with the Conventional Plaque-Reduction Neutralization Test
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Lee Hee-Jung
Min Kyung-Il
Park Ki-Hoon
Choi Hyo-Jung
Kim Min-Kyoung
Ahn Chi-Young
Hong Young-Jin
Kim Young-Bong
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Abstract
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We previously reported the development of a neutralization assay system for evaluating Japanese Encephalitis Virus (JEV) neutralizing antibody (NAb) using pseudotyped-JEV (JEV- PV). JEV-PV-based neutralization assay offers several advan-tages compared with the current standard plaque-reduc-tion neutralization test (PRNT), including simplicity, safety, and speed. To evaluate the suitability of the JEV-PV assay as new replacement neutralization assay, we compared its repeatability, reproducibility, specificity, and correlated its results with those obtained using the PRNT. These analyses showed a close correlation between the results obtained with the JEV-PV assay and the PRNT, using the 50% plaque re-duction method as a standard for measuring NAb titers to JEV. The validation results met all analytical acceptance criteria. These results suggest that the JEV-PV assay could serve as a safe and simple method for measuring NAb titer against JEV and could be used as an alternative approach for assaying the potency of JEV neutralization.
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KEYWORD
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Japanese encephalitis virus, neutralizing antibody, pseudovirus, PRNT
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